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Comparison between import emulsifier samples and LipidMate on many indexes
Time£º3.10.2011  Website's Browsing Times £º3270¡¡¡¼BLack¡½
 

Comparison between import emulsifier samples and LipidMate on many indexes

 

MATERIALS

Experimental material

1. Import emulsifier sample (Europe), without date of manufacture and batch number.

2. The emulsifier sample was supplied by Hangzhou King Techina Feed Co., Ltd. The trade name is LipidMate. The date of manufacture is Nov 24th, 2008. The batch number is 08112401.

3. Plant oil: Fook Lam Moon edible soybean oil (decoloration), first-grade, date of manufacture is Jan 8th, 2008.

Solution preparation

1. Preparation of artificial gastric juice:

Added 800ml of water and 10g of protease into 16.4ml of dilute hydrochloric acid, mixed, then, added water to 100ml. (2005 of Chinese Pharmacopoeia, page 72 appendix)

2. Preparation of artificial intestinal juice:

Dissolve 6.8g of monopotassium phosphate was in 500ml of water, adjusted PH value to 6.8 with NaOH solution (0.1mol/L), 10g of pancreatin was dissolved with water, then mixed two solution and added water to 1000ml. (2005 of Chinese Pharmacopoeia, page 158 appendix)

Method of comparison

1. Comparison of appearance

The comparison of two types of emulsifiers on appearance indexes (colour and lustre, particle size, flowability, uniformity, moisture content) by visual inspection.

2. Comparison of emulsion effect

In vitro method for simulating the emulsion: added 0.5g of emulsifier into 50ml of artificial gastric juice or intestinal juice, stirred 2min, added 50ml of edible oil, stirred 2min. The effect and stability of emulsion were observed at 0min and 2min.

RESULTS

Comparison of appearance

Put 0.5g of emulsifier on the blue table, the colour and lustre, particle size, flowability, uniformity, dissolubility and moisture content were observed and recorded by taking photos. The result was shown in the table.  

Indexes

Emulsifier of Belgium

LipidMate

Colour and lustre

Faint yellow

white

Particle size

Bigger

smaller

flowability

Bad, with blocking

Good, without blocking

uniformity

uneven

uniform

Moisture content

higher

lower

Figure 1: the comparison of two types of emulsifiers on appearance

Comparison of emulsion effect

1. Comparison of emulsion effect in artificial gastric juice

In strict accordance with in vitro method for simulating the emulsion: added 0.5g of emulsifier into 50ml of artificial gastric juice, stirred 2min and took photos immediately, as emulsion effect of 0min (figure 2). The results shown that the oil floated and gathered again with using emulsifier of Belgium, but emulsified oil was milk white and uniform with using LipidMate. The emulsified oil using emulsifier of Belgium layering obviously, after stand 30min (figure 3). There was an obvious cut-off rule between oil and intestinal juice, after stand 120min (figure 4), there was an obvious boundary line between oil and water, the oil dissociated from the emulsifier system, after stand 15d (figure 5), the oil dissociated completely from the emulsifier system, there was no difference between proportion of dissociated oil and additive amount of oil, that is to say, the emulsifier had no emulsifying ability after standing 15d.

After using LipidMate, the system of oil and gastric juice turned milk white, without big oil drop, standing 30min, 120min and 15d, there was no visible oil drop in emulsifier oil, but there was free gastric juice at the bottom of beaker because the gastric juice exceed the requirement of emulsified oil.

The water phase must have participated in the emulsification process, or else lipid couldn¡¯t be emulsified. In the forming process of emulsified oil, the oil was emulsified to small oil drop and became stable emulsification system, the water phase molecule is necessary for divising fully the small emulsified oil drop. So the breaking of emulsion won¡¯t occur and no free oil in the emulsification system.

 

Figure 2 artificial gastric juice emulsion of 0min      Figure 3 artificial gastric juice emulsion of 30min

 

Figure 4 artificial gastric juice emulsion of 120min       Figure 5 artificial gastric juice emulsion of 15d

2. Comparison of emulsion effect in artificial intestinal juice

In strict accordance with in vitro method for simulating the emulsion: added 0.5g of emulsifier into 50ml of artificial intestinal juice, stirred 2min and took photos immediately, as emulsion effect of 0min (figure 6). Standing 30min, 120min and 15d, took photos, the photos were figure 7, 8, and 9 respectively. The results were the same with emulsification of artificial gastric juice.

 

Figure 6 artificial intestinal juice emulsion of 0min    Figure 7 artificial intestinal juice emulsion of 30min

 

Figure 8 artificial intestinal juice emulsion of 120min   Figure 9 artificial intestinal juice emulsion of 15d

ANALYSIS

At present, the most of emulsifiers have the question of the stability of emulsified oil, break the emulsions rapidly after emulsification, break the emulsions rapidly. When we develop LipidMate, the main question was the stability of emulsification system and sustained time as long as possible. The results show that the emulsified oil without dissociative oil when placed more than 2 weeks.

Comparison between import emulsifier samples and LipidMate on many indexes